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In ARPE19 cells, Hif1a was significantly downregulated by administration of Garcinia extract regardless of the presence garcinia cambogia right dosage absence of CoCl2 Figure 2A The downstream genes of HIFs such as Vegfa, Bnip3, and Pdk1, were upregulated by CoCl2 and significantly downregulated by Garcinia extract administration Figure 2B D Similarly, Hif1a was downregulated by administration of Garcinia extract in 661W cells Figure 2E CoCl2-induced upregulation of Vegfa was also downregulated by Garcinia extract administration in 661W cells Figure 2F Expression of other downstream genes of HIFs showed a tendency to be downregulated as well as Vegfa Figure 2G, HCA also downregulated Hif1a and the downstream genes in ARPE19 cells Figure 3A D and 661W cells Figure 3E H Both Garcinia extract and HCA suppressed HIF-1 protein expression increased by CoCl2 administration in ARPE19 cells Figure 4A, and 661W cells Figure 4C, Hif1 and the downstream genes were downregulated by Garcinia extract administration.

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contained in this document, marked by brackets, has been omitted and filed separately with the Securities and Exchange Commission pursuant to Rule 406 of the Securities Act of 1933, as amended. contained in this document, marked by brackets, has been omitted and filed separately with the Securities and Exchange Commission pursuant to Rule 406 of the Securities Act of 1933, as amended.

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